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目的 探讨强荧光氮硫共掺杂碳点(N, S-CDs)的主要成分和实际发光来源——2种柠檬酸衍生物荧光小分子，5-氧代-3,5-二氢噻唑-3,2-吡啶-3,7-二羧酸(TPA)和5-氧代-3,5-二氢噻唑-3,2-吡啶-7-羧酸(TPCA)的生物毒性，为生物应用安全性提供实验依据。方法 利用斑马鱼模型评价TPA和TPCA生物毒性，包括观察代谢途径、器官病变及确定中毒阈值。结果 TPA通过肾和肠道代谢，急性毒性表现为肝变小和卵黄囊吸收延迟，毒性阈值为1 000μmol/L;TPCA通过肠道代谢，急性毒性表现为肝变性，毒性阈值为20 mmol/L,是TPA的20倍，其毒性远远小于TPA。结论 本研究明确了TPA与TPCA在斑马鱼体内的毒性差异及中毒阈值，对其在生物体内的应用安全性提供了关键实验依据。
Abstract：Objective To evaluate the biotoxicological safety of two fluorescent citric acid derivatives, namely 5-oxo-3,5-dihydro-2 H-thiazolo[3,2-a]pyridine-3,7-dicarboxylic acid(TPA) and 5-oxo-3,5-dihydro-2 H-thiazolo[3,2-a]pyridine-7-carboxylic acid(TPCA), which are the primary constituents and the actual luminophores of highly fluorescent nitrogen-sulfur co-doped carbon dots(N, S-CDs). The objective is to provide experimental evidence for the safety of biological applications. Methods In this study, the biological toxicity of TPA and TPCA was evaluated using zebrafish model, which included examining their metabolic pathways, organ lesions, and toxicity thresholds. Results It was found that TPA was metabolized through the renal and intestinal, the acute toxic behaviors of zebrafish were reduced liver and delayed yolk sac absorption, the toxic threshold value was 1000 μmol/L. TPCA was metabolized through the gut, and the acute toxicity behavior of zebrafish is mainly manifested as hepatic degeneration, the toxic threshold value is 20 mmol/L, 20 times that of TPA, which was far less toxic than TPA. Conclusion This research provides crucial experimental evidence for the biosafety of TPA and TPCA, informing their potential application in vivo.
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基本信息:
DOI：10.16421/j.cnki.1002-3127.2025.06.004
中图分类号:X171.5
引用信息:
[1]陈咏梅,魏巍,张瑗,等.利用斑马鱼模型评价有机荧光发光物质的毒性[J].毒理学杂志,2025,39(06):404-411.DOI:10.16421/j.cnki.1002-3127.2025.06.004.
基金信息:
国家重点研发计划(2022YFE0104200); 陕西省科技发展计划(2020KWZ-006); 外国专家项目(G20200241017)